Antioxidative Defense System in Pisum sativum Roots Exposed to Heavy Metals (Pb, Cu, Cd, Zn)

摘要

Heavy metals (Cd, Pb, Cu, Zn) absorbed by roots brought about oxidative stress conditions through ROS production (O_2~-, H_2O_2) for pea plants cultivated hydroponically for 96 h on a Hoagland medium with the addition of 50 uM: CdCl_2, Pb(NO_3)_2, CuSO_4, and ZnSO_4. We shows, using laser ablation ICP MS, that Cd, Cu, Pb, and Zn elements are located along a cross-section through the roots of pea plants. We observed increasing activities of antioxidative enzymes (SOD, CAT, GR) in oxidative stress conditions. We have shown changes in redox state (GSH/GSSG) in pea root grown with Pb, Cu, Cd, and Zn.