Indirect semiquantitative determination of p34~cdc levels in G_1 and G_2 cells of the carbohydrate-starved root meristems in vicia faba var. minor

摘要

In eukaryotes, the 34kDa kinase (p34) encoded by the cdc2 gene is a key regulator of both the onset of DNA synthesis (G_1 to S phase transition) and the onset of mitosis (g_2 to M phase transition). Using mouse anti-human PSTAIRE and FITC- labelled goat antibodies, indirect semiquantitative determination of p34~cdc2 levels was performed in meristematic cells from the control (intact) and excised, carbohydrate-starved main roots of Vicia faba var. Minor.