PICLORAM INDUCED SOMATIC EMBRYOGENESIS IN CHICKPEA (CICER ARIETINUM L)

摘要

Complete plantlets were regenerated via somatic embryogenesis in the widely cultivated chickpea variety BG256. Leaf explants, from 13 day-old axenic seedlings yielded somatic embryos with high efficiency during dark incubation on MS medium supplemented with B-5 vitamins, 0.25 mg/l each of 2,4-D and picloram, and 0.1 mg/l BAP. A modified B-5 medium with 2 mg/l BAP and 0.1 mg/l IAA was found suitable for maturation of the embryos. The well-formed embryos germinated on basal B-5 medium supplemented with 0.25 mg/l BAP and developed into healthy plantlets on basal B-5 medium. Hardened plantlets grew into normal, fertile pod bearing plants upon transfer to soil. [References: 21]