n nnnFlowering plants control energy allocation to their photosystemsin response to light quality changes. This includes the phosphorylationand migration of light-harvesting complex II (LHCII) proteins(state transitions or short-term response) as well as long-termalterations in thylakoid composition (long-term response orLTR). Both responses require the thylakoid protein kinase STN7.Here, we show that the signaling pathways triggering state transitionsand LTR diverge at, or immediately downstream from, STN7. Bothresponses require STN7 activity that can be regulated accordingto the plastoquinone pool redox state. However, LTR signalingdoes not involve LHCII phosphorylation or any other state transitionstep. State transitions appear to play a prominent role in floweringplants, and the ability to perform state transitions becomescritical for photosynthesis in Arabidopsis thaliana mutantsthat are impaired in thylakoid electron transport but retaina functional LTR. Our data imply that STN7-dependent phosphorylationof an as yet unknown thylakoid protein triggers LTR signalingevents, whereby an involvement of the TSP9 protein in the signalingpathway could be excluded. The LTR signaling events then ultimatelyregulate in chloroplasts the expression of photosynthesis-relatedgenes on the transcript level, whereas expression of nuclear-encodedproteins is regulated at multiple levels, as indicated by transcriptand protein profiling in LTR mutants.展开▼
机译:ABSTRACTn FONT> TH> TR> TABLE> n
n TOP n <字体颜色= 464c53>抽象 FONT> n 介绍 n 结果 n 讨论 n 结论 n 方法 n 参考资料 n FONT> TH> TR> TABLE> n nnn开花植物响应光质量的变化,控制向其光系统 SUP>的能量分配。这包括光捕获复合物II(LHCII)蛋白 SUP>的磷酸化和迁移(状态转换或短期响应)以及长期 类囊体组成中的SUP>变化(长期响应或 SUP> LTR)。这两种反应都需要类囊体蛋白激酶STN7。在这里,我们显示了触发状态转换的信号通路 SUP>和LTR在STN7或紧邻STN7的下游发散。这两个 SUP>响应都需要STN7活性,可以根据 SUP>调节其对醌醌池的氧化还原状态。但是,LTR信号 SUP>不涉及LHCII磷酸化或任何其他状态转换 SUP>步骤。状态过渡似乎在开花的 SUP>植物中起着重要作用,而状态转化的能力对于拟南芥 I>突变体的光合作用变得至关重要。 > SUP>在类囊体电子传输中受损,但保留 SUP>功能性LTR。我们的数据表明,迄今未知的类囊体蛋白的STN7依赖性磷酸化 SUP>触发LTR信号 SUP>事件,从而使TSP9蛋白参与信号 SUP >路径可以排除。 LTR信号事件最终在叶绿体中最终 SUP>在转录水平上调节与光合作用相关的 SUP>基因的表达,而核编码的 SUP>蛋白的表达则是LTR突变体中的转录物 SUP>和蛋白质谱分析表明,其在多个水平上受到调节。 SUP>
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Dipartimento di Produzione Vegetale, Università degli studi di Milano c/o Parco Tecnologico Padano Via Einstein, 26900 Lodi, Italy|Dipartimento di Scienze Biomolecolari e Biotecnologie, Università degli studi di Milano, 20133 Milan, Italy;
Lehrstuhl für Botanik, Department Biologie I, Ludwig-Maximilians-Universit?t, 82152 Planegg-Martinsried, Germany;
Lehrstuhl für Botanik, Department Biologie I, Ludwig-Maximilians-Universit?t, 82152 Planegg-Martinsried, Germany|Mass Spectrometry Unit, Department Biologie I, Ludwig-Maximilians-Universit?t, 82152 Planegg-Martinsried, Germany;
Lehrstuhl für Botanik, Department Biologie I, Ludwig-Maximilians-Universit?t, 82152 Planegg-Martinsried, Germany;
Lehrstuhl für Pflanzenphysiologie, Friedrich-Schiller-Universit?t, 07443 Jena, Germany;
Lehrstuhl für Botanik, Department Biologie I, Ludwig-Maximilians-Universit?t, 82152 Planegg-Martinsried, Germany;
Department of Biotechnology, Intercollegiate Faculty of Biotechnology of University of Gdansk and Medical University of Gdansk, 80-822 Gdansk, Poland;
Lehrstuhl für Botanik, Department Biologie I, Ludwig-Maximilians-Universit?t, 82152 Planegg-Martinsried, Germany;
Lehrstuhl für Botanik, Department Biologie I, Ludwig-Maximilians-Universit?t, 82152 Planegg-Martinsried, Germany;
Lehrstuhl für Botanik, Department Biologie I, Ludwig-Maximilians-Universit?t, 82152 Planegg-Martinsried, Germany;
Lehrstuhl für Pflanzenphysiologie, Friedrich-Schiller-Universit?t, 07443 Jena, Germany;
Lehrstuhl für Botanik, Department Biologie I, Ludwig-Maximilians-Universit?t, 82152 Planegg-Martinsried, Germany;