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>Shoot Na+ Exclusion and Increased Salinity Tolerance Engineered by Cell Type-Specific Alteration of Na+ Transport in Arabidopsis
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Shoot Na+ Exclusion and Increased Salinity Tolerance Engineered by Cell Type-Specific Alteration of Na+ Transport in Arabidopsis
n nnnSoil salinity affects large areas of cultivated land, causingsignificant reductions in crop yield globally. The Na+ toxicityof many crop plants is correlated with overaccumulation of Na+in the shoot. We have previously suggested that the engineeringof Na+ exclusion from the shoot could be achieved through analteration of plasma membrane Na+ transport processes in theroot, if these alterations were cell type specific. Here, itis shown that expression of the Na+ transporter HKT1;1 in themature root stele of Arabidopsis thaliana decreases Na+ accumulationin the shoot by 37 to 64%. The expression of HKT1;1 specificallyin the mature root stele is achieved using an enhancer trapexpression system for specific and strong overexpression. Theeffect in the shoot is caused by the increased influx, mediatedby HKT1;1, of Na+ into stelar root cells, which is demonstratedin planta and leads to a reduction of root-to-shoot transferof Na+. Plants with reduced shoot Na+ also have increased salinitytolerance. By contrast, plants constitutively expressing HKT1;1driven by the cauliflower mosaic virus 35S promoter accumulatedhigh shoot Na+ and grew poorly. Our results demonstrate thatthe modification of a specific Na+ transport process in specificcell types can reduce shoot Na+ accumulation, an important componentof salinity tolerance of many higher plants.展开▼
机译:ABSTRACTn FONT> TH> TR> TABLE> n
n TOP n <字体颜色= 464c53>抽象 FONT> n 介绍 n 结果 n 讨论 n 方法 n 参考文献 n FONT> TH> TR> TABLE> n nnn土壤盐分会影响大片耕地,导致 SUP>信号全球农作物产量下降。许多农作物的Na + SUP>毒性 SUP>与芽中Na + SUP> SUP>的过量积累有关。先前我们曾提出,可以通过质膜Na + SUP> SUP>的交替实现从芽中排除Na + SUP>的工程 SUP> / SUP>传输过程在 SUP>根中,如果这些更改是特定于单元格类型的。在这里, SUP>显示了>拟南芥 I>的 SUP>成熟根碑中Na + SUP>转运蛋白HKT1; 1的表达>将芽中的Na + SUP>积累 SUP>降低37%至64%。 HKT1; 1 I>特别是 SUP>在成熟根碑中的表达是通过使用增强子捕获 SUP>表达系统实现的,从而实现特异的强表达。芽中的 SUP>效应是由HKT1; 1介导的Na + SUP>进入石cells根细胞的流入量增加引起的。植株中的 SUP>导致Na + SUP>的根茎转移 SUP>减少。 Na + SUP>的芽生植物减少的盐分 SUP>的耐性也增加。相比之下,由花椰菜花叶病毒35S启动子驱动的组成型表达 HKT1; 1 I> SUP>的植物积累了 SUP>高芽Na + SUP>并且成长不佳。我们的结果表明 SUP>在特定 SUP>细胞类型中对特定Na + SUP>转运过程的修饰可以减少芽Na + SUP>积累,是许多高等植物耐盐性的重要组成部分。 SUP>
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Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, United Kingdom|Australian Centre for Plant Functional Genomics, University of Adelaide, SA 5064, Australia;
School of Agriculture, Food, and Wine, University of Adelaide, SA 5064, Australia;
Australian Centre for Plant Functional Genomics, University of Adelaide, SA 5064, Australia|School of Agriculture, Food, and Wine, University of Adelaide, SA 5064, Australia;
Australian Centre for Plant Functional Genomics, University of Adelaide, SA 5064, Australia|School of Agriculture, Food, and Wine, University of Adelaide, SA 5064, Australia;
Australian Centre for Plant Functional Genomics, University of Adelaide, SA 5064, Australia|School of Agriculture, Food, and Wine, University of Adelaide, SA 5064, Australia;
Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, United Kingdom;
Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, United Kingdom;
Australian Centre for Plant Functional Genomics, University of Adelaide, SA 5064, Australia|School of Agriculture, Food, and Wine, University of Adelaide, SA 5064, Australia;