Identification and Characterization of Maize and Barley Lsi2-Like Silicon Efflux Transporters Reveals a Distinct Silicon Uptake System from That in Rice
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>Identification and Characterization of Maize and Barley Lsi2-Like Silicon Efflux Transporters Reveals a Distinct Silicon Uptake System from That in Rice
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Identification and Characterization of Maize and Barley Lsi2-Like Silicon Efflux Transporters Reveals a Distinct Silicon Uptake System from That in Rice
n nnnSilicon (Si) uptake has been extensively examined in rice (Oryzasativa), but it is poorly understood in other gramineous crops.We identified Low Silicon Rice 2 (Lsi2)-like Si efflux transportersfrom two important gramineous crops: maize (Zea mays) and barley(Hordeum vulgare). Both maize and barley Lsi2 expressed in Xenopuslaevis oocytes showed Si efflux transport activity. Furthermore,barley Lsi2 was able to recover Si uptake in a rice mutant defectivein Si efflux. Maize and barley Lsi2 were only expressed in theroots. Expression of maize and barley Lsi2 was downregulatedin response to exogenously applied Si. Moreover, there was asignificant positive correlation between the ability of rootsto absorb Si and the expression levels of Lsi2 in eight barleycultivars, suggesting that Lsi2 is a key Si transporter in barley.Immunostaining showed that maize and barley Lsi2 localized onlyat the endodermis, with no polarity. Protein gel blot analysisindicated that maize and barley Lsi2 localized on the plasmamembrane. The unique features of maize and barley Si influxand efflux transporters, including their cell-type specificityand the lack of polarity of their localization in Lsi2, indicatethat these crops have a different Si uptake system from thatin rice.展开▼
机译:ABSTRACTn FONT> TH> TR> TABLE> n
n TOP n <字体颜色= 464c53>抽象 FONT> n 介绍 n 结果 n 讨论 n 方法 n 参考文献 n FONT> TH> TR> TABLE> n nnn硅(Si)的吸收已在水稻中进行了广泛研究( Oryza SUP>苜蓿 I>),但在其他禾本科作物中了解甚少。 SUP>我们从两种重要禾本科作物中鉴定出了低硅水稻2(Lsi2)状的硅外排转运蛋白 SUP>。 :玉米( Zea mays I>)和大麦 SUP>( Hordeum vulgare I>)。在非洲爪蟾卵母细胞中表达的玉米和大麦Lsi2均表现出Si外排转运活性。此外, SUP>大麦Lsi2能够恢复水稻外源突变的 SUP>水稻突变体中的硅吸收。玉米和大麦 Lsi2 I>仅在 SUP>根中表达。玉米和大麦 Lsi2 I>的表达下调 SUP>,这是由于外源施硅引起的。此外,八个大麦中 SUP>的根吸收硅的能力与 Lsi2 I>的表达水平之间存在显着的正相关。 / SUP>品种,表明Lsi2是大麦中的关键Si转运蛋白。 SUP>免疫染色显示,玉米和大麦Lsi2仅位于 SUP>内胚层,没有极性。蛋白质凝胶印迹分析 SUP>表明玉米和大麦Lsi2位于血浆 SUP>膜上。玉米和大麦硅流入 SUP>和外排转运蛋白的独特特征,包括它们的细胞类型特异性 SUP>,以及它们在Lsi2中定位的缺乏极性,表明 SUP>这些农作物与水稻中的 SUP>具有不同的硅吸收系统。 SUP>
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