Impact of graphene oxide and silver diamine fluoride in comparison to photodynamic therapy on bond integrity and microleakage scores of resin modified glass ionomer cement to demineralized dentin

摘要

Aim: To evaluate the impact of graphene oxide (GO) and silver diammine fluoride (SDF) in comparison to photodynamic therapy (PDT) on adhesive bond integrity and microleakage scores of resin-modified glass ionomer cement (RMGIC) to demineralized dentin. Methods: 80 non-carious and atraumatic third molars were mounted inside the segments of polyvinyl pipes. Exposure, homogenization, and polishing of dentinal tubules was performed. 20 specimens were left unconditioned and classified a group 4. Exposure of acetic acid was done to 60 specimens and randomly allocated into 3 groups. Group 1 specimens were treated with 0.25 % GO; group 2 specimens were treated with 3.8 % SDF; and group 3 specimens were exposed to PDT. The bonding of all samples to RMGIC was performed by light curing and universal testing machine was utilized for testing shear bond strength (SBS). For failure modes, an optical microscope at 40x magnification was used to observe the debonded interfaces. For microleakage, all the specimens were submerged in 2% methylene blue for 1 day and observed under a digital microscope. The post hoc Tukey and analysis of variance (ANOVA) were used to examine SBS. A p-value of ?0.05 was considered statistically significant. Results: Group 2 showed the highest bond strength (3.8 % SDF + RMGIC + DD ? 15.27 ? 2.19 MPa). Groups 1 (0.25 % GO + RMGIC + DD ? 14.11 ? 3.82) and 2 showed comparable results of SBS, which was significantly higher when compared with other research groups (p 0.05). According to the post hoc Tukey test, the SBS scores of treatment groups exhibited statistically significant values as compared to the control group (p 0.05). Group 2 samples showed the least microleakage mean score (0.8 ? 0.42), followed by group 1 specimens (1.02 ? 0.88). The comparable microleakage mean scores were demonstrated by the samples in group 1 and 2 (p 0.01). According to one-way ANOVA, a significant difference was observed in microleakage scores among experimental groups (p 0.01). Conclusion: Improved bond strength and marginal microleakage scores were demonstrated by the demineralized dentin treated with 0.25 % graphene oxide and 3.8 % silver diamine fluoride when bonded to resin-modified glass ionomer cement. The application of methylene blue photosensitizer for photodynamic therapy undermined the adhesive bond strength applied on demineralized dentin.