Effects of indomethacin, celecoxib and meloxicam on glutathione, malondialdehyde and myeloperoxidase in rat gastric tissue

摘要

In the present study, we examined the effects of indomethacin, celecoxib and meloxicam on the stomach and on levels of glutathione (GSH), malondialdehyde (MDA) and activity of myeloperoxidase (MPO) in gastric tissues of rats. The ulcer area was 31.1 ± 10.7 mm2 in indomethacin-treated rats (20 mg kg-1) and 17.3 ± 38 mm2 in meloxicam-treated rats (7.5 mg kg-1). Celecoxib, at a dose of 100 mg kg-1, did not cause any damage in the rat gastric tissue. GSH levels were 1.6 ± 0.3, 6 ± 0.7 and 3.3 ± 0.4 nmol/g protein in the indomethacin-, celecoxib- and meloxicam-treated groups, respectively. MPO activities were 27.6 ± 2.4, 12.3 ± 1.5 and 14.9 ± 0.7 U/g protein in the indomethacin-, celecoxib- and meloxicam-treated rats, respectively. MDA levels were 184.8 ± 40.9, 81.47 ± 15.4, 113 ± 18.1 mol/g protein, respectively. In the control group, GSH, MDA levels and activity of MPO were 6.5 ± 1 nmol/g protein, 62.1 ± 11.1 mol/g protein, 11.2 ± 1.5 U/g protein, respectively.Taking these data into consideration, we propose that the depletion of GSH in gastric mucosa, as well as inhibition of PG synthesis, may play an important role in development of gastric mucosal injury. In addition, there is a parallelism between the increase of MPO activity, levels of MDA and severity of gastric mucosal injury.