Neuroprotective effects of an extract from the inflamed skin of rabbits inoculated with vaccinia virus on glutamate-induced neurotoxicity in cultured neuronal cell line

摘要

Objective: Protein-free extracts from the inflamed skin of rabbits inoculated with vaccinia virusn(RosemorgenH and NeurotropinH) are widely employed to combat chronic pain and treat allergicnconditions in human subjects in Japan. However, the pharmacologic mechanisms ofnRosemorgenH and NeurotropinH remain unclear.nMethods: In this study, we examined the effects of RosemorgenH on L-glutamic acid (Glu)-ninduced cell death in N18-RE-105 neural cell line, which only possessed non-N-methyl-Daspartaten(NMDA)-type receptors.nResults: There were many large cytoplasmic cells and elongation of fivers in phosphate-bufferednsaline (PBS) additional group without Glu. In PBS and Glu simultaneous additional group, thensurvival ratio was decrease significantly compared with PBS alone group. Moreover, there werendead cells which did not have cytoplasm and aggregated nucleus. The Glu-induced cell death ofnN18-RE-105 cells was inhibited by both pre-treatment (24 hours before Glu treatment) andnsimultaneous treatment with RosemorgenH. There were many large cytoplasmic cells andnelongation of fivers in RosemorgenH group.nDiscussion: From this finding in N18-RE-105 cells, RosemorgenH was concluded to inhibit Gluinducedncell death via non-NMDA type receptors. One of the pharmacologic mechanisms ofnRosemorgenH has been clear. These results suggest that RosemorgenH depresses allodynia and chronicnpain through interaction with non-NMDA type receptors. [Neurol Res 2008; 30: 430–434]