The BRCA2 homologue Brh2 nucleates RAD51 filament formation at a dsDNA-ssDNA junction

摘要

The BRCA2 tumour suppressor(1) is essential for the error- free repair of double- strand breaks ( DSBs) in DNA by homologous recombination(2,3). This is mediated by RAD51, which forms a nucleoprotein filament with the 30 overhanging single- stranded DNA ( ssDNA) of the resected DSB, searches for a homologous donor sequence, and catalyses strand exchange with the donor DNA(4). The 3,418- amino- acid BRCA2 contains eight similar to 30- amino-acid BRC repeats that bind RAD51 ( refs 5, 6) and a similar to 700- amino-acid DBD domain that binds ssDNA(7). The isolated BRC and DBD domains have the opposing effects of inhibiting(8,9) and stimulating recombination(7), respectively, and the role of BRCA2 in repair has been unclear. Here we show that a full- length BRCA2 homologue ( Brh2) stimulates Rad51- mediated recombination at substoichiometric concentrations relative to Rad51. Brh2 recruits Rad51 to DNA and facilitates the nucleation of the filament, which is then elongated by the pool of free Rad51. Brh2 acts preferentially at a junction between double- stranded DNA ( dsDNA) and ssDNA, with strict specificity for the 30 overhang polarity of a resected DSB. These results establish a BRCA2 function in RAD51- mediated DSB repair and explain the loss of this repair capacity in BRCA2- associated cancers.