Super-resolution microscopy

摘要

For imaging beyond the diffraction limit, to resolve tiny features in cells for example, researchers rely on tagging the imaged object with fluorescent chromophores or on other microscopy techniques that do not exploit fluorescence but are much less sensitive. A team from the Department of Chemistry and Chemical Biology at Harvard has now developed an alternative approach known as stimulated emission microscopy, incorporating techniques previously used in other multiphoton microscopies. The sensitivity of the method was demonstrated in applications including label-free microvascular imaging and monitoring lacZ gene expression with a chromogenic reporter.