Nanog, Pou5fl and SoxB1 activate zygotic gene expression during the maternal-to-zygotic transition

摘要

这篇论文识别出了在胚胎形成过程中负责合子程序的启动的关键因子。在受精之后，母体因子引导发育，并在母体一合子过渡时触发合子基因组的激发。Antonio Giraldez及同事采用"功能丧失"分析、高吞吐量转录组测序和核糖体足迹法识别出了多能因子Nanog、 Pou5f1和SoxB1在斑马鱼胚胎的合子转录的启动及母方转录体的清除中所起的重要作用。%After fertilization, maternal factors direct development and trigger zygotic genome activation (ZGA) at the maternal-to-zygotic transition (MZT). In zebrafish, ZGA is required for gastrulation and clearance of maternal messenger RNAs, which is in part regulated by the conserved microRNA miR-430. However, the factors that activate the zygotic program in vertebrates are unknown. Here we show that Nanog, Pou5fl (also called Oct4) and SoxBl regulate zygotic gene activation in zebrafish. We identified several hundred genes directly activated by maternal factors, constituting the first wave of zygotic transcription. Ribosome profiling revealed that nanog, soxl9b and pou5fl are the most highly translated transcription factors pre-MZT. Combined loss of these factors resulted in developmental arrest before gastrulation and a failure to activate ＞75% of zygotic genes, including miR-430. Our results demonstrate that maternal Nanog, Pou5fl and SoxB1 are required to initiate the zygotic developmental program and induce clearance of the maternal program by activating miR-430 expression.