Inhibitory effect of magnetic iron-oxide nanoparticles on the pattern of expression of lanosterol 14α-demethylase (ERG11) in fluconazole-resistant colonising isolate of Candida albicans

摘要

Fluconazole-resistantCandida albicansis a big scary reality. The authors assessed the antifungal effects of magnetic iron-oxide nanoparticles on fluconazole-resistant colonising isolate ofC. albicansand determined the expression ofERG11gene, protein sequence similarity and ergosterol content.C. albicansisolates were characterised and fluconazole resistance is recognised using World Health Organization's WHONET software. Susceptibility testing of magnetic iron-oxide nanoparticles against fluconazole-resistant colonising isolate ofC. albicanswas performed according to Clinical and Laboratory Standards Institute guidelines. The expression patterns ofERG11and protein sequence similarity were investigated. Ergosterol quantification has been used to gauge the antifungal activity of magnetic iron-oxide nanoparticles. The findings indicated that 93% ofC. albicansisolates were resistant to fluconazole. Magnetic iron-oxide nanoparticles were presented activity against fluconazole-resistant colonising isolate ofC. albicanswith minimum inhibitory concentration at 250-500 mu g/ml. The expression level ofERG11gene was downregulated in fluconazole-resistant colonising isolate ofC. albicans. The results revealed no differences in fluconazole-resistant colonising isolate ofC. albicansby comparison with ERG11 reference sequences. Moreover, significant reduction was noted in ergosterol content. The findings shed a novel light on the application of magnetic iron-oxide nanoparticles in fighting against resistantC. albicans.