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首页> 外文期刊>Molecular imaging >Rh-I-UEA-1 Polymerized Liposomes Target and Image Adenomatous Polyps in the APC~(Min/+) Mouse Using Optical Colonography
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Rh-I-UEA-1 Polymerized Liposomes Target and Image Adenomatous Polyps in the APC~(Min/+) Mouse Using Optical Colonography

机译：Rh-I-UEA-1聚合脂质体靶向和图像腺瘤性息肉在APC〜（Min / +）小鼠中的光学结肠成像

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摘要

Mutated adenomatous polyposis coli (APC) genes predispose transformations to neoplasia, progressing to colorectal carcinoma. Early detection facilitates clinical management and therapy. Novel lectin-mediated polymerized targeted liposomes (Rh-I-UEA-1), with polyp specificity and incorporated imaging agents were fabricated to locate and image adenomatous polyps in APC~(Min/+) mice. The biomarker α-L-fucose covalently joins the liposomal conjugated lectin Ulex europaeus agglutinin (UEA-1), via glycosidic linkage to the polyp mucin layer. Multispectral optical imaging (MSI) corroborated a global perspective of specific binding (rhodamine B 532 nm emission, 590-620 nm excitation) of targeted Rh-I-UEA-1 polymerized liposomes to polyps with 1.4-fold labeling efficiency. High-resolution coregistered optical coherence tomography (OCT) and fluorescence molecular imaging (FMI) reveal the spatial correlation of contrast distribution and tissue morphology. Freshly excised APC~(Min/+) bowels were incubated with targeted liposomes (UEA-1 lectin), control liposomes (no lectin), or iohexol (Omnipaque) and imaged by the three techniques. Computed tomographic quantitative analyses did not confirm that targeted liposomes more strongly bound polyps than nontargeted liposomes or iohexol (Omnipaque) alone. OCT, with anatomic depth capabilities, along with the coregistered FMI, substantiated Rh-I-UEA-1 liposome binding along the mucinous polyp surface. UEA-1 lectin denotes u-L-fucose biomarker carbohydrate expression at the mucin glycoprotein layer; Rh-I-UEA-1 polymerized liposomes target and image adenomatous polyps in APC~(Min/+) mice.
机译：突变的腺瘤性息肉病大肠埃希菌（APC）基因易于转化为赘生物，发展为大肠癌。早期发现有助于临床管理和治疗。制造具有息肉特异性和结合显像剂的新型凝集素介导的聚合靶向脂质体（Rh-1-UEA-1），以定位和成像APC〜（Min / +）小鼠的腺瘤性息肉。生物标记物α-L-岩藻糖通过糖苷键连接到息肉粘蛋白层上，共价结合脂质体结合的凝集素Ulex europaeus凝集素（UEA-1）。多光谱光学成像（MSI）证实了靶向Rh-I-UEA-1聚合脂质体与息肉的特异性结合（罗丹明B 532 nm发射，激发590-620 nm激发）的全局透视，具有1.4倍的标记效率。高分辨率共注册光学相干断层扫描（OCT）和荧光分子成像（FMI）揭示了对比度分布和组织形态的空间相关性。将新鲜切下的APC〜（Min / +）肠与靶向脂质体（UEA-1凝集素），对照脂质体（无凝集素）或碘海醇（Omnipaque）孵育，并通过三种技术进行成像。计算机断层扫描定量分析未确认靶向脂质体比单独的非靶向脂质体或碘海醇（Omnipaque）更牢固地结合息肉。具有解剖深度功能的OCT以及共同注册的FMI，证实了沿粘液息肉表面的Rh-1-UEA-1脂质体结合。 UEA-1凝集素表示粘蛋白糖蛋白层的u-L-岩藻糖生物标志物碳水化合物表达； Rh-1-UEA-1聚合脂质体靶向并成像APC〜（Min / +）小鼠的腺瘤性息肉。

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  • 来源
    《Molecular imaging》 |2011年第4期|p.305-316|共12页
  • 作者

    Celeste A. Roney; Biying Xu; Jianwu Xie; Shuai Yuan; Jeremiah Wierwille; Chao-Wei Chen; Yu Chen; Gary L. Griffiths; Ronald M. Summers;

  • 作者单位

    Imaging Biomarkers and Computer-Aided Diagnosis Laboratory, Radiology and Imaging Sciences Department, Clinical Center, and The Imaging Probe Development Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD and Fischell Department of Bioengineering, University of Maryland,College Park, MD;

    Imaging Biomarkers and Computer-Aided Diagnosis Laboratory, Radiology and Imaging Sciences Department, Clinical Center, and The Imaging Probe Development Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD and Fischell Department of Bioengineering, University of Maryland,College Park, MD;

    Imaging Biomarkers and Computer-Aided Diagnosis Laboratory, Radiology and Imaging Sciences Department, Clinical Center, and The Imaging Probe Development Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD and Fischell Department of Bioengineering, University of Maryland,College Park, MD;

    Imaging Biomarkers and Computer-Aided Diagnosis Laboratory, Radiology and Imaging Sciences Department, Clinical Center, and The Imaging Probe Development Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD and Fischell Department of Bioengineering, University of Maryland,College Park, MD;

    Imaging Biomarkers and Computer-Aided Diagnosis Laboratory, Radiology and Imaging Sciences Department, Clinical Center, and The Imaging Probe Development Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD and Fischell Department of Bioengineering, University of Maryland,College Park, MD;

    Imaging Biomarkers and Computer-Aided Diagnosis Laboratory, Radiology and Imaging Sciences Department, Clinical Center, and The Imaging Probe Development Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD and Fischell Department of Bioengineering, University of Maryland,College Park, MD;

    Imaging Biomarkers and Computer-Aided Diagnosis Laboratory, Radiology and Imaging Sciences Department, Clinical Center, and The Imaging Probe Development Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD and Fischell Department of Bioengineering, University of Maryland,College Park, MD;

    Imaging Biomarkers and Computer-Aided Diagnosis Laboratory, Radiology and Imaging Sciences Department, Clinical Center, and The Imaging Probe Development Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD and Fischell Department of Bioengineering, University of Maryland,College Park, MD;

    Imaging Biomarkers and Computer-Aided Diagnosis Laboratory, Radiology and Imaging Sciences Department, Clinical Center, and The Imaging Probe Development Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD and Fischell Department of Bioengineering, University of Maryland,College Park, MD;

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  • 入库时间 2022-08-18 00:39:16

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