Reply to Comment on 'Deletion of btnl, an orthologue of CLN3,increases glycolysis and perturbs amino acid metabolism in the fission yeast model of Batten disease'

摘要

Pearce and Padilla-Lopez are incorrect in stating that the btnl mutant strain is auxotroph with respect to leucine. The Schizosaccharomyces pombe btn1Δ strain used in the recently published metabolomic study (Pears et al. 2010)6 is one that has been used in several previous studies (Gachet et al. 2005, Codlin et al. 2008a, 2008b, Codlin and Mole 2009).1-3,5 It was made by replacing the endogenous btnl gene with S. cerevisiae leu2 (which complements S, pombe leul-31) (Gachet et al. 2005).5 In these experiments we used wildtype strain 972.6 Both strains can grow in minimal media, and do not require the supply of leucine since they are able to synthesize it themselves.