Sample preparation approaches for qualitative and quantitative analysis of lipid-derived electrophile modified proteomes by mass spectrometry

摘要

Lipid-derived electrophile (LDE) modifications, which are covalent modifications of proteins by endogenousLDEs, are essential types of protein posttranslational modifications. LDE modifications alter the proteinstructure and regulate their biological processes in cells. LDE modifications of proteins are also closelyassociated with several diseases and function as potential biomarkers for clinical diagnosis. The crucial stepin studying the LDE modifications is to enrich the LDE modified proteins/peptides from complex biologicalsamples with high efficiency and high selectivity and quantify modified proteins/peptides with high accuracy.In this review, we summarize the recent progress in MS-based proteomic technologies to globally identify andquantify LDE modified proteomes, mainly focusing on discussing the qualitative and quantitative technologies.