Piecing together MS data

摘要

There was a time when protein identificationnwas a simple matter of isolating a proteinnband from a polyacrylamide gel, digestingnit with enzymes, and performing amino acidnsequencing on the resulting peptides. Butnas proteomic technologies have improved,nthe variety of proteins to be isolated and characterizednhas increased dramatically to thenpoint where researchers are nowntrying to analyze hundreds to thousandsnof spots on a single 2-D gel.