Simultaneous measurement of dissolved oxygen concentration and velocity field in microfluidics using oxygen-sensitive particles

摘要

This paper reports a technique for measuring the velocity and dissolved oxygen concentration (DOC) fields simultaneously in a micro-scale water flow using oxygen-sensitive particles (OSP) and a conventional microparticle image velocimetry method. The OSP were fabricated using a dispersion polymerization method by synthesizing platinum (Ⅱ) octaethyporphyrin (PtOEP) with polystyrene, and used as tracer particles and oxygen sensors. An ultraviolet light-emitting diode with a wavelength of 385 nm was used as the excitation light source, and phosphorescence images of OSP were captured on a CMOS high-speed camera. The interrogation window concept was used to measure the DOC in water from the dispersed phosphorescence intensity distribution of OSP. The Stern-Volmer equations in the interrogation windows were obtained from in situ calibration. Water containing OSP with DOC values of 0 and 100 % were injected into a Y-shaped microchannel using a double loading syringe pump. The velocity and DOC field over the entire channel area were quantified.