Ultrastructure of human pheochromocytoma cells cultured for long periods

摘要

We zconducted ultrastructural analysis of human pheochromocytoma(PC)cells maintained in primary cul- ture of about 10 months. The cells were first isolated by the enzymatic treatment of a surgically resected tissue speci- men obtained from at 37-year-old man with PC, a condition which is characterized by elevated blood levels of adrena- line and noradrenaline. It was found that noradrenaline production in the medium continued until the 90th day of culture(1330 pg/ml). The production level decreased to 20pg/ml on the 180th day, and to 18pg/ml on the 30th day.