Synthesis and bio-applications of carbohydrate-gold nanoconjugates with nanoparticle and nanolayer forms

摘要

Carbohydrate-conjugated gold nanopartides (GNPs) and gold nanolayers (GNLs), which have recently attracted increasing attention as innovative nano-biomaterials, were successfully synthesized using carbohydrate thiosemicarbazones in an aqueous N-methylmorpholine N-oxide (NMMO) system. This system can dissolve various types of carbohydrates including structural polysaccharides, e.g., cellulose. We observed that oxidative NMMO solvent unexpectedly promoted the immediate reduction of [Au~Ⅲ Cl_4]~- species to nanosized metallic Au°, that is GNP. One possible mechanism is that the chloride ligand was oxidized to chlorate via the formation of a hypochlorite intermediate. Site-selective S-labeling of sugar reducing ends with thiosemicarbazide may have enabled regulated self-assembly immobilization to the surfaces of GNPs and GNLs. The carbohydrate-gold nanoconjugates possess unique nanoarchitectures and biofunctions for carbohydrate-cell interactions. Novel and simple approaches for the structural design of carbohydrate-decorated GNPs and GNLs in the NMMO system have potential to inspire a new phase in glyco-biomaterials eneineerine.