Immobilization of Acidithiobacillus ferrooxidans on sulfonated microporous poly(styrene-divinylbenzene) copolymer with granulated activated carbon and its use in bio-oxidation of ferrous iron

摘要

The immobilization efficiencies of Acidithiobacillus ferrooxidans cells on different immobilization matrices were investigated for biooxidation of ferrous iron (Fe~(2+)) to ferric iron (Fe~(3+)). Six different matrices were used such as the polyurethane foam (PUF), granular activated carbon (GAC), raw poly(styrene-divinylbenzene) copolymer (rawSDVB), raw poly(styrene-divinylbenzene) copolymer with granular activated carbon (rawSDVB-GAC), sulfonated poly(styrene-divinylbenzene) copolymer (sulfSDVB) and sulfonated poly(styrene-divinylbenzene) copolymer with granular activated carbon (sulfSDVB-GAC). The sulfSDVB-GAC polymer showed the best performance for Fe~(2+) biooxidation. It was used at packed-bed bioreactor and the kinetic parameters were obtained. The highest Fe~(2+) biooxidation rate (R) was found to be 4.02 g/L h at the true dilution rate (D_t) of 2.471/h and hydraulic retention time (τ) of 0.4 h. The sulfSDVB-GAC polymer was used for the first time as immobilization material for A ferrooxidans for Fe~(2+) biooxidation.