Poly(ε-caprolactone)/poly(glycerol sebacate) electrospun scaffolds for cardiac tissue engineering using benign solvents

摘要

Electrospun fibers based on combinations of poly(epsilon-caprolactone) (PCL) and poly(glycerol sebacate) (PGS) have been studied for applications in cardiac tissue engineering. The aim of the present study is to demonstrate the fabrication of PCL and PGS prepolymer or mildly crosslinked PGS by electrospinning using less toxic solvents, like acetic acid, as opposed to conventional solvents such as chloroform or dichloromethane. The morphological and physiochemical properties and degradation susceptibility of the fiber mats were determined. A cell study using ST2 cells was performed to assess cytocompatibility. Neat PCL and PCL/PGS blends showed defect-free microstructures, whereby the average fiber diameter increased with the addition of PGS (0.8 +/- 0.3 mu m and 1.3 +/- 0.7 mu m, respectively). The mechanical properties were tested at 23 degrees C and 37 degrees C and showed higher values compared to native human myocardium. Degradation studies revealed a fast PGS degradation but the mildly cross-linked PGS resulted in acidification of the degradation medium. The biocompatibility was significantly increased when the samples were disinfected in 70% v/v ethanol in comparison to ultra-violet light disinfection. PCL/PGS fibers fabricated with acetic acid proved to be potentially suitable for application in cardiac tissue engineering based on their mechanical properties and biodegradability.