Developmental Disturbances Caused by Polychlorinated Biphenyls in Zebrafish (Brachydanio rerio)

摘要

A Selection of four PCB congeners (PCB#60, PCB#104, PCB#173 and PCB#190) and estradiol 17-βwere tested on zebrafish for their effect on early embryo development. The females were kept separately in 21 aquaria and injected with the different compounds (1μmol kg~-1) or peanut oil. Ten days after injection the females were mated with uninjected male zebrafish and the eggs were collected and kept at 28 deg. C. It was observed that injection of estradiol 17-β(1 μmol kg~-1 w. w.) and PCB#104 (2,2＇4,6,6＇-tetraCB) resulted in high mortality before hatching. Injection of PCB#60 (2, 3, 4, 4＇-teraCB) also resulted in high mortality and this was found to be due to impaired hatching.