Biosynthesis of Platinum Nanoparticles by Escherichia coli MC4100: Can Such Nanoparticles Exhibit Intrinsic Surface Enantioselectivity?

摘要

The biomanufacture of two types of platinumnbionanoparticle (bioNP) using Escherichia coli MC4100n(1% and 20% by mass metal loading) together with a methodnfor both liberating the nanoparticles (NPs) from the bacterialnlayer and their subsequent critical cleaning is reported. Thenpossibility of an enantiomeric excess of chiral kink sitesnforming on the surface of the Pt nanoparticles produced by thenbacteria was investigated using the electrooxidation of D- and Lglucosenas the chiral probe. Transmission electron microscopy revealed that the Pt bioNPs (after recovery and cleaning) werentypically 2.3 ± 0.7 nm (1% loading) and 4.5 ± 0.7 nm (20% loading) in diameter. The D- and L-glucose electrooxidation measurementsndid not give rise to any chiral response using either of the Pt bioNPs types but did display differing CV profiles. Thisnsuggested that the overall surface morphology of each bioNP could be controlled by the degree of metal loading but that nonenantiomeric excess of intrinsically chiral surface kink sites was present.