Estimation of bacterial production through the coupling of (methyl 3H) Thymidine incorporation and several parameters over a Microcystis aeruginosa bloom in a hypereutrophic reservoir

摘要

The coupling of the changes in bacterial quantitative and metabolic aspects during Microcystis aeruginosa bloom conditions together with several environmental parameters was studied in the hypereutrophic Villerest reservoir, France. Bacterial abundance varied from 5.20 to 21.28 × 106 bacteria-mL−1, while bacterial biomass ranged between 75 and 507 Μg C mL−1. These results confirmed the highly eutrophic status of the Villerest reservoir. The relative quantitative importance of attached bacteria increased as Microcystis proliferated. Methyl-3H incorporation and D-(U-14C) glucose uptake varied from 8.99 to 60.57 × 106 cells-mL−1, and 0.016 to 1.587 Μg CL−1 h−1, respectively. Correlations between several abiotic and biotic parameters showed that phytoplankton regulated bacterial growth by releasing organic carbon which is directly uptaken by heterotrophic bacteria. In the hypolimnion, the sedimentation of decaying algae represented a substantial substrate for bacterial growth.