Simulation of protein adsorption in a batchwise affinity chromatography with a modified rate model

摘要

A rate model was adapted to simulate the dynamics of protein adsorption. This model takes axial dispersion and film mass transfer into account where there is a nonlinear adsorption isotherm for protein. The model equations were solved with the application of orthogonal collocation method on finite elements. The model is validated with experimental adsorption of urokinase in a batchwise column chromatographic process. Adsorption kinetics and isotherm were measured in a batchwise operation. With the assumption of back mixing at the column inlet, the effect of the different flow pattern on the concentration change inside the column can be simulated with the rate model.