Self-Cleavable Stimulus Responsive Tags for Protein Purification without Chromatography

摘要

The need for simple methods to purify recombinant proteins is increasingly urgent and is driven by two technological imperatives.At the laboratory level,the advent of proteomics and high-throughput structural biology has created an urgent demand for simple methods to express and purify recombinant proteins.Although the use of affinity tags in combination with chromato-graphic separation has gained widespread use for purification of recombinant proteins,affinity tags have several problems for lab-scale purification of recombinant proteins:(1)they require chromatographic separation to achieve high purity,which limits their application in a high-throughput setting,and(2)when implemented in batch mode,the level of purity is typically insufficient for structural biology.