Laminar Flow-Based Electrochemical Microreactor for Efficient Regeneration of Nicotinamide Cofactors for Biocatalysis

摘要

This communication reports a microfluidic electrochemical method for efficient regeneration of cofactors such as NADH for biocatalysis.The use of more environmentally friendly and stereo-selective enzymatic transformations for the industrial synthesis of fine chemicals such as pharmaceutical intermediates,food additives,and cosmetics continues to gain interest.For example,oxidoreduc-tases are enzymes that catalyze the asymmetric reduction of carbonyl groups to alcohols and amines or promote the oxygenation of C-H bonds in the presence of nicotinamide cofactors such as NAD(P)H.Stoichiometric use of these cofactors,however,is cost prohibitive,leaving in situ regeneration of the cofactor as one of the few remaining alternatives.Yet,the development of efficient methods of cofactor regeneration is one of the long-standing challenges that prevent the more widespread use of biocatalysis.