Orientation Control of Fluorescence Resonance Energy Transfer Using DNA as a Helical Scaffold

摘要

Fluorescence resonance energy transfer (FRET) between an excited fluorescence donor and a ground state fluorescence acceptor has been widely used to study the structure and dynamics of molecules in the gas phase,solution,and solid state.FRET has been employed in studies of DNA duplex formation,duplex structure,and duplex protein binding.Elegant studies by Clegg et al.and by Hurley and Tor using complementary duplexes possessing donor and acceptor probes separated by a variable number of base pairs have shown that the FRET efficiency is dependent upon the vector distance between the chromophores,rather than the number of base pairs.Jovin employed an ingenious FRET experiment to establish the helical handedness of short duplexes.