In Vitro Selection of Aptamers with Affinity for Neuropeptide Y Using Capillary Electrophoresis

摘要

Systematic evolution of ligands by exponential enrichment (SELEX) is a process for isolating high affinity ligands,referred to as aptamers,from random-sequence nucleic acid libraries.A number of reviews describing the SELEX process have been published.In general,a large pool of random-sequence ssDNA or RNA is generated.Sequences with affinity for the target are enriched through some selection process,usually nitrocellulose filtration or affinity chromatography.