Building Addressable Libraries:The Use of Electrochemistry for Spatially Isolating a Heck Reaction on a Chip

摘要

As part of a broader effort to build addressable chip-based molecular libraries,scientists at CombiMatrix have been utilizing active semiconductor chips that incorporate arrays of individually addressable microelectrodes to synthesize molecules.In this way,each unique set of molecules in a library can be located proximal to a unique electrode or set of electrodes that can in turn be used to monitor their behavior.This is accomplished by coating the micro-electrode-containing chips with a porous polymer and then utilizing the electrodes to both attach monomers to the chips and then generate reagents capable of performing reactions on the monomers.The reagents generated at any given electrode are confined to the area surrounding the electrode by placing a substrate in the solution above the electrode that consumes the reagent.