Inorganic-Organic Hybrid Luminescent Binary Probe for DNA Detection Based on Spin-Forbidden Resonance Energy Transfer

摘要

We have designed, synthesized, and successfully demonstrated the utility of SF-RET to target DNA using a hybrid inorganic-organic probe set with a ruthenium complex as donor and Cy5 as acceptor. With TRE, the delayed fluorescence of the Cy5 acceptor due to SF-RET can be employed to distinguish target signal, from intense, but short-lived, background emission such as cell autofluorescence. Additionally, Ru complexes possess tunable absorption and emission profiles, by using different ligands, an advantage for use with fluorescence microscopes. SF-RET may be useful for the detection of DNA and RNA in highly fluorescent media in vitro and in vivo. Further studies are in progress to evaluate the effectiveness of these probes inside living cells.