A Rapid Flow Mixer with 11 -μs Mixing Time Microfabricated by a Pulsed-Laser Ablation Technique: Observation of a Barrier-Limited Collapse in Cytochrome c Folding

摘要

The exponential kinetics was observed for the initial folding phase of cyt c, which strongly suggested the barrier-limited collapse. The developed mixer can be combined with various spectroscopic and scattering techniques for the analysis of protein folding and other fast events.