Site-Site Interactions Enhances Intramolecular Electron Transfer in Streptomyces coelicolor laccase

摘要

The multicopper oxidases (MCOs) catalyze the four-electron reduction of O_2 to H_2O. Here we report that the intramolecular electron transfer (ET) rates in the small bacterial laccase isolated from Streptomyces coelicolor (SLAC) markedly depend on the reduction state of the enzyme, possibly reflecting site-site interactions so far not observed in other MCOs. SLAC has recently been shown to have structural and reactivity features distinct from those of other laccases. It was therefore of interest to investigate the intramolecular ET rates in SLAC.