Higher Sensitivity through Selective ~(13)C Excitation in Solid-State NMR Spectroscopy

摘要

In solid-state NMR (ssNMR) spectroscopy, it has become feasible to determine the structures of small crystalline proteins (<100 residues) at atomic resolution. This is an important development for structural biology, as it potentially makes accessible many macromolecular systems that have proven to be unsuited to diffraction methods or solution-state NMR analysis. However, while the first successful ssNMR studies of membrane proteins and amyloid samples have indeed recently been reported, many protein systems of interest are still difficult or impossible to tackle because of the low intrinsic sensitivity of ssNMR.