Microfluidic Device for Single-Molecule Experiments with Enhanced Photostability

摘要

Single-molecule Forster resonance energy transfer (smFRET) experiments can elucidate biomolecular structure and dynamics at a resolution beyond the reach of common ensemble techniques. The quality of the smFRET signal depends on the number of emitted photons, which is generally limited by photobleaching of the fluorescent dyes. Because a substantial fraction of photobleaching pathways are mediated by molecular oxygen, a common remedy is the addition of an enzymatic oxygen scavenging system.