Electrostatic Redesign of the [Myoglobin, Cytochrome b_5] Interface To Create a Well-Defined Docked Complex with Rapid Interprotein Electron Transfer

摘要

Interactions between macromolecules are fundamental to most of biology. The goal of understanding and controlling protein-protein interactions has inspired numerous efforts to modulate the binding within high-affinity complexes of known structure, although most alterations serve to weaken binding. To begin such efforts at the other extreme, with a pair of proteins that bind weakly, and by altering them to create a well-defined, highly functional complex, offers a different level of challenge. We here report dramatic progress in creating such a complex between the weakly interacting physiological electron-transfer (ET) partners, myoglobin (Mb) andrncytochrome b_5(cyt b_5).