A Hydrogen Peroxide-Responsive Hyperpolarized ~(13)C MRI Contrast Agent

摘要

We report a new reaction-based approach for the detection of hydrogen peroxide (H_2O_2) using hyperpolarized ~(13)C magnetic resonance imaging (~(13)C MRI) and the H_2O_2-mediated oxidation of (X-ketoacids to carboxylic acids. ~(13)C-Benzoylformic acid reacts selectively with H_2O_2 over other reactive oxygen species to generate ~(13)C-benzoic acid and can be hyperpolarized using dynamic nuclear polarization, providing a method for dual-frequency detection of H_2O_2. Phantom images collected using frequency-specific imaging sequences demonstrate the efficacy of this responsive contrast agent to monitor H_2O_2 at pre-clinical field strengths. The combination of reaction-based detection chemistry and hyperpolarized ~(13)C MRI provides a potentially powerful new methodology for non-invasive multi-analyte imaging in living systems.