A Catalyst Selection Protocol That Identifies Biomimetic Motifs from β-Hairpin Libraries

摘要

Assaying a solid-phase library of histidine-containing β-hairpin peptides by a reactive tagging scheme in organic solvents selects for catalysts that reproduce the strategies used by His-based enzyme active sites to accelerate acyl- and phosphonyl-transfer reactions. Rate accelerations (k_(rel)) in organic solvents of up to 2.4 × 10~8 are observed.