BIOCHEMICAL CHANGES AND GROWTH RESPONSES INDUCED BY CADMIUM IN BEAN SEEDLINGS (PHASEOLUS VULGARIS L.)

摘要

Cadmium (Cd) is one of the widespread environmental pollutants which has toxicity effect and its consumption by animals and humans with their diet can lead to serious diseases. The Present study was carried out in order to investigate the effect of Cd stress on the growth, SOD activity, Proline and ABA content in bean seedlings. In this experiment, 25 bean seeds genotypes were germinated and grown in laboratory conditions consisting of solution CdCl_2 with concentration of 4mg/lit and distilled water (as a control). The seedlings were harvested after 10days and the germination percentage, root and shoot length, fresh weight, sod activity, praline and ABA content were recorded. Application cadmium caused a significant reduction in germination percentage 9.9%, fresh weight 43.2%, root and shoots elongation 83.9% and 66.3%, respectively as compared with that of control while Cd treated plant showed an increase in Sod activity (20.79%), Praline (20.71%) and ABA (41.57%) content compared to control. Based on our results we concluded that, decrease in seed germination and seedling growth by Cd exposure can be related to this toxicity and oxidative stress. Also, the present results revealed that the increase in sod activity, praline and ABA content causes to prevent the occurrence of oxidative damage under Cd stress.