Enzymatic Removal of Carboxyl Protecting Groups.2.Cleavage of the Benzyl and Methyl Moieties

摘要

Enzymes are versatile reagents for the efficient removal of methyl and benzyl protecting groups.An esterase from Bacillus subtilis(BS2)and a lipase from Candida antarctica(CAL-A)allow a mild and selective removal of these moieties in high yields without affecting other functional groups.The problem of functional group incompatibility is especially acute in the design and construction of poly-functional organic molecules such as peptides,oligosac-charides,glycopeptides,and nucleotides and in general natural products,which often require a scaffold of protecting groups.Methyl and benzyl esters are the most common of the carboxylic protecting groups because of their ease of introduction.