Two-Photon Induced Uncaging of a Reactive Intermediate.Multiphoton In Situ Detection of a Potentially Valuable Label for Biological Applications

摘要

Two-photon induced Wolff rearrangement of a terphenyl diazoketone 1 was achieved by using focused laser pulses of 532 nm from a Q-switched Nd:YAG laser.The nonfluorescent terphenyl diazoketone 1 was transformed into a fluorescent ester derivative 4,which can be detected in situ using the focused laser pulses at 532 nm.Laser power dependence studies show that the Wolff rearrangement is induced by two-photon absorption of the terphenyl diazoketone 1,but suggests that more than two photons of 532 nm are involved (a multiphoton process) in excitation of the ester derivative 4.