Expression of Inositol 1, 4 , 5- trisphosphate Receptor mRNA in Myocardium of Spontaneous Hypertension Rats and Cultured Vascular Smooth Muscle Cells of Rats

摘要

Objective To investigate expression of inositol 1, 4, 5-trisphosphate receptor (IP_3R) mRNA on sacroplasmic reticular in myocardium of spontaneous hypertension rats (SHRs) and cultured vascular smooth muscle cells (VSMC) of rats and effects of perindopril and urapidil on them. Methods SHRs were orally given perindopril (1.0 mg · kg~(-1) · d~(-1)) or urapidil (15 mg · kg~(-1) · d~(-1)) for 24 weeks, respectively. Expression of LP_3R mRNA was examined by semi-quantitative reverse transcription polymers chain reaction (RT-PCR) using three oligonuclotide primers for each subtype of IP_3R with β-actin as internal label. Results All, subtypes of IP_3R were expressed in myocardium of SHR,WKY and, cultured, VSMC. Expression of IP_3R mRNA in left ventricle of SHR was markedly enhanced. Urapidil could down-regulate expression of IP_3R- Ⅰ and IP_3R- Ⅲ, perindopril, slightly increased expression of IP_3R- Ⅱ and decreased expression of IP_3R- Ⅰ and IP_3R- Ⅲ in myocardium of SHR. Conclusion Our results suggest that expression of IP_3R mRNA in cardiovascular system could be regulated by urapidil and perindopril.