Altered expression of adipose differentiation-related protein gene in placental tissue of pre-eclampsia

摘要

Objective: To investigate the altered expression of lipid metabolism-related gene adipose differentiation-related protein (ADRP) in pre-eclampsia. Methods: Semi-quantitative RT-PCR and Western blotting were used to validate the altered expression of ADRP gene between pre-eclamptic placentas (pre-eclampsia group) and normotensive placentas (control group) respectively. In situ hybridization (ISH) was used to localize ADRP mRNA in pre-eclamptic placentas. Results: There was a significant difference in the levels of placental ADRP mRNA between pre-eclampsia group and control group (1.98 ± 0.50 υs 1.09 ± 0.20, P < 0.01). Western blotting showed that placentas both in pre-eclampsia group and control group expressed the special ADRP band at 48.1 kD. The relative levels of ADRP protein in pre-eclampsia group were significantly higher than those of control group (0.40 ± 0.19 υs 0.19 ± 0.09, P < 0.01). ADRP mRNA was diffusely distributed in pre-eclamptic placentas. Their positive staining existed in cytoplasm of trophoblast. Conclusion: Abnormal expression of ADRP gene in pre-eclamptic placenta may be associated with the pathogenesis of pre-eclampsia.