Exact and approximate distributions of protein and mRNA levels in the low-copy regime of gene expression

摘要

Gene expression at the single-cell level incorporates reaction mechanisms which are intrinsically stochastic as they involve molecular species present at low copy numbers. The dynamics of these mechanisms can be described quantitatively using stochastic master-equation modelling; in this paper we study a generic gene-expression model of this kind which explicitly includes the representations of the processes of transcription and translation. For this model we determine the generating function of the steady-state distribution of mRNA and protein counts and characterise the underlying probability law using a combination of analytic, asymptotic and numerical approaches, finding that the distribution may assume a number of qualitatively distinct forms. The results of the analysis are suitable for comparison with single-molecule resolution gene-expression data emerging from recent experimental studies.