Scaffold-free approach produces neocartilage tissue of similar quality as the use of HyStem (TM) and Hydromatrix (TM) scaffolds

摘要

Numerous biomaterials are being considered for cartilage tissue engineering, while scaffold-free systems have also been introduced. Thus, it is important to know do the scaffolds improve the formation of manufactured neo-cartilages. This study compares scaffold-free cultures to two scaffold-containing ones. Six million bovine primary chondrocytes were embedded in HyStem (TM) or HydroMatrix (TM) scaffolds, or suspended in scaffold-free chondrocyte culture medium, and then loaded into agarose gel supported culture well pockets. Neocartilages were grown in the presence of hypertonic high glucose DMEM medium for up to 6 weeks. By the end of culture periods, the formed tissues were analyzed by histological staining for proteoglycans (PGs) and type II collagen, gene expression measurements of aggrecan, Sox9, procollagen alpha(1)(II), and procollagen alpha(2)(I) were performed using quantitative RTPCR, and analyses of PG contents and structure were conducted by spectrophotometric and agarose gel electrophoretic methods. Histological stainings showed that the PGs and type II collagen were abundantly present in both the scaffold-free and the scaffold-containing tissues. The PG content gradually increased following the culture period. However, the mRNA expression levels of the cartilage-specific genes of aggrecan, procollagen alpha(1)(II) and Sox9 gradually decreased following culture period, while procollagen alpha(2)(I) levels increased. After 6-week-cultivations, the PG concentrations in neocartilage tissues manufactured with HyStem (TM) or HydroMatrix (TM) scaffolds, and in scaffold-free agarose gel-supported cell cultures, were similar to native cartilage. No obvious benefits could be seen on the extracellular matrix assembly in HyStem (TM) or HydroMatrix (TM) scaffolds cultures.