EXPERIMENTALLY DESIGNED, VALIDATED HPLC SIMULTANEOUS DETERMINATION OF PRIDINOL AND DICLOFENAC IN THEIR COMBINED PHARMACEUTICAL FORMULATIONS, WHICH ALLOWS LIMITING DICLOFENAC RELATED COMPOUND A

摘要

The development and validation of an HPLC method for the determination of pridinol and diclofenac in their combined formulations and the simultaneous limit testing of diclofenac related compound A is described. The separation was performed on a C₁₈ column. Experimental design and response surface strategies were employed for optimizing detection wavelength (225 nm) and mobile phase composition [MeOH:2-propanol:phosphate buffer (50 mM, pH 5.5), 48:9:43 (v/v/v), 1 mL min−1], and for validation purposes. The method was successfully applied to the quality control of commercial brands of tablets and capsules. Found impurity levels were below 0.1% (LOQ = 0.02%). Stressed samples were also evaluated.View full textDownload full textKeywordsdiclofenac, diclofenac related compound A, experimental design, HPLC determination, pridinol, validationRelated var addthis_config = { ui_cobrand: "Taylor & Francis Online", services_compact: "citeulike,netvibes,twitter,technorati,delicious,linkedin,facebook,stumbleupon,digg,google,more", pubid: "ra-4dff56cd6bb1830b" }; Add to shortlist Link Permalink http://dx.doi.org/10.1080/10826076.2010.519239