Correlation between Cyclin A Gene Expression in Adult Patients with Acute Leukemia and Drug Resistance

摘要

In order to investigate the relationship between the expression of cyclin A and drug resistance in adult patients with acute leukemia (AL) , the mRNA expression of cyclin A, mdr1, Top Ⅱ_α bcl-2 was detected in 64 adult patients with AL and 20 normal controls by semi-reverse transcription polymerse chain reaction (semi-RT-PCR). It was found that the cyclin A and Top Ⅱ_α mRNA expression levels in drug resistant group were significantly lower than in sensitive group (P< 0. 01). Under the same experimental condition no cyclin A mRNA expression was detectable in all normal controls. The mdrl and bcl-2 mRNA expression levels in resistant group were significantly higher than in sensitive group (P<0. 01). cyclin A and Top Ⅱ_α gene expression levels were closely correlated (r_s = + 0. 794, P=0. 000, n = 64) in all AL patients, but cyclin A was not correlated with mdrl and bcl-2 gene expression levels. In drug resistant group there was a negative correlation between the gene expression levels of cyclin A and mdrl (r_s = - 0. 337, P = 0. 029). The 10 AL patients with positive lower expression of both cyclin A and Top Ⅱ_α were all resistant to drugs. Logistic regression of Binary analysis showed the correlation between the lower expression of cyclin A and drug resistance. It was concluded that lower expression of cyclin A gene might be an unfavorable prognostic factor for patients with AL, and detection of both cyclin A and Top Ⅱ_α gene expression would predict drug resistance in AL patients.