Ad/CMV- hTGF-β1 Treats Rabbit Intervertebral Discs Degeneration in Vivo

摘要

To investigate therapeutic efficiency of Ad/CMV- hTGF-β1 gene for rabbit intervertebral disc degeneration model. 60 Japanese white rabbits were selected to form the L5-L6 Anterior-Lateral- Anulus-Fibrosus-Incision-Induced model in order to simulate human intervertebral disc degeneration. 36 rabbits, whose corresponding intervertebral discs were injected with 20 μl ( 10 X 10~6 pfu)of Ad/CMV- hTGF-β1 gene, constituted the therapy group, 12 were injected with 20 μl (10 X 10~6 pfu)of Ad/CMV-LacZ gene as comparison group, while 12 were only injected with equivalent capacity of saline for empty comparison group. 3 weeks after injection, examples were taken for investigation of HE staining. MR1. Western Blotting and immunohistochemical research TGF-β1. Wide distribution of TGF-β1 was detected by immunohistochemical research in the degenerated an-nulus fibrosus after injection. Western Blotting research showed significant increase of TGF-β1 content in intervertebral discs treated with TGF-β1 gene than comparison groups. MRI signal transformed from low to comparatively high and that intervertebral disc pathological degree improved, Ad/CMV- hTGF-β1 gene transfection is a potential method to increase TGF-β1 content and reverse intervertebral disc degeneration.