Effect of hydraulic retention time on the biodegradation of complex phenolic mixture from simulated coal wastewater in hybrid UASB reactors

摘要

This study describes the feasibility of anaerobic treatment of complex phenolics mixture from a simulated synthetic coal wastewater using four identical 13.5 L (effective volume) bench scale hybrid up-flow anaerobic sludge blanket (HUASB) (combining UASB + anaerobic filter) reactors at four different hydraulic retention times (HRT) under mesophilic (27 ± 5 ℃) conditions. Synthetic coal wastewater with an average chemical oxygen demand (COD) of 2240 mg/L and phenolics concentration of 752 mg/L was used as substrate. The phenolics contained phenol (490 mg/L); m-, o-, p-cresols (123.0, 58.6,42 mg/L); 2,4-, 2,5-, 3,4- and 3,5-dimethyl phenols (6.3,6.3,4.4 and 21.3 mg/L) as major phenolic compounds. The study demonstrated that at optimum HRT, 24 h, and phenolic loading rate of 0.75 g COD/(m~3-d), the phenolics and COD removal efficiency of the reactors were 96% and 86%, respectively. Bio-kinetic models were applied to data obtained from experimental studies in hybrid UASB reactor. Grau second-order multi-component substrate removal model was best fitted to the hybrid UASB reactor. The second-order substrate removal rate constant (k_2(s)) was found as 1.72 h~(-1) for the hybrid reactor treating complex phenolic mixture. Morphological examination of the sludge revealed rod-type Methanoathrix-like, cells to be dominant on the surface.